Nature Communications (Jul 2022)

Etv2 regulates enhancer chromatin status to initiate Shh expression in the limb bud

  • Naoko Koyano-Nakagawa,
  • Wuming Gong,
  • Satyabrata Das,
  • Joshua W. M. Theisen,
  • Tran B. Swanholm,
  • Daniel Van Ly,
  • Nikita Dsouza,
  • Bhairab N. Singh,
  • Hiroko Kawakami,
  • Samantha Young,
  • Katherine Q. Chen,
  • Yasuhiko Kawakami,
  • Daniel J. Garry

DOI
https://doi.org/10.1038/s41467-022-31848-6
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 17

Abstract

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The embryonic limb bud is known to be patterned by a Shh morphogen gradient, though how Shh expression is activated remains less clear. Here the authors show that Etv2 acts as a pioneer transcription factor to mediate accessibility of the ZRS enhancer and initiate Shh expression.