Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses
Isabel Barranco,
Camila P. Rubio,
Asta Tvarijonaviciute,
Heriberto Rodriguez-Martinez,
Jordi Roca
Affiliations
Isabel Barranco
Department of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano dell’Emilia, Bologna, Italy
Camila P. Rubio
Department of Animal and Food Science, School of Veterinary Science, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain
Asta Tvarijonaviciute
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, Spain
Heriberto Rodriguez-Martinez
Department of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, 58185 Linköping, Sweden
Jordi Roca
Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Murcia, Spain
The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (n = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 °C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (p p p p p < 0.05).
