Study of the Effect of Carob (Ceratoniasiliqua L.) Extract Activity as Antibiotic from UTI

Abstract

Escherichia coli bacterial cells have been collected and selected from(30) patients (most found strain) in urine samples 25 (83.3 %) suffering from infection of urinary tract laid down in Hashimiyaha teaching hospital, Babylon during a period from Novembertwo thousand sixty to February two thousand seventy. The isolated strain diagnosis is confirmed withVitek2 system apparatus which performed to identify species level ofEscherichia coli isolates.To evaluate the antimicrobialaction of the ethanol extract of Carob (CeratoniasiliquaL.) podsonlyas well as in mixture with certain drugs (64µg /ml ampicillin, 32µg /ml gentamicin, 128µg /ml amikacin,8µg /ml clindamycin.) as the wide usageantibiotics in the treatment of UTI bacterial infectionswhich has led to the emergence and spread of resistant strains. Many studies showed that the efficacy of antimicrobials can be improved by combining them with crude plant extracts. The antimicrobial activity of the ethanol extract of pods of Carob(CeratoniasiliquaL.)alone as well as in mixture with some standard antimicrobials has been evaluated using well diffusion methodwhich demonstrates an in-vitro antibacterial activity of the tested extracts against E. Coli bacteria. A combination of the tested extracts( concentration 100%,50%) with antibacterial has increased that activity of the tested antimicrobials.The results revealed the importance of Carob plant extracts when associated with antibiotics to regulatorresistance E. Coli bacteria that developed as adanger to human health