Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity
Dang Diem Hong,
Le Thi Thom,
Nguyen Cam Ha,
Ngo Thi Hoai Thu,
Hoang Thi Minh Hien,
Luu Thi Tam,
Nguyen Manh Dat,
Tran Mai Duc,
Nguyen Van Tru,
Nguyen Thi Minh Hang,
Ranga Rao Ambati
Affiliations
Dang Diem Hong
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Le Thi Thom
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Nguyen Cam Ha
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Ngo Thi Hoai Thu
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Hoang Thi Minh Hien
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Luu Thi Tam
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Nguyen Manh Dat
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Tran Mai Duc
Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nha Trang 57100, Vietnam
Nguyen Van Tru
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Nguyen Thi Minh Hang
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
Ranga Rao Ambati
Department of Biotechnology, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation for Science, Technology and Research (Deemed to Be University), Vadlamudi, Guntur 522213, India
Fucoxanthin extracted and purified from Vietnamese Sargassum oligocystum Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes were investigated. Fucoxanthin isolated from Vietnamese S. oligocystum showed no cytotoxic effects; moreover, it exhibited AChE inhibitory activity (with an IC50 value of 130.12 ± 6.65 μg mL−1) and antioxidant activity (with an IC50 value of 3.42 ± 0.15 mg mL−1). At concentrations of 50 and 100 µg mL−1, fucoxanthin provided protection against amyloid β-protein fragment 25–35-induced neurotoxicity in a C6 neuronal cell line, and the survival of C6 cells was higher than 81.01% and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis suggested that the neuroprotective effect of fucoxanthin resulted from regulation of the gene expression of antioxidant enzymes (CAT and GPx) and ER pathways (caspase-3 and Bax), as well as the promotion of expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5), and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the seaweed S. oligocystum in Vietnam is a potential feedstock source for the production of health foods that exert neuroprotective effects.
