Immunohistochemical investigation of histopathological changes in the kidney tissue of mussel-fed rats

Abstract

Purpose: The aim of this study was to determine the histopathological changes that the mussels, which serve as important filters of the sea, cause in rat kidney tissue. Materials and Methods: In our study, 24 female (Wistar albino 6-10 week old rats) rats were used. First group (n: 6): fed with standard rat food, second group (n: 6): fed everyday with 4/5 mussels + 1/5 standard rat food; third group (n: 6): fed 4/5 mussels + 1/5 standard rat food every two days; Fourth group (n: 6): were formed with 4/5 mussels + 1/5 standard rat food to be given every three days. After the routine histopathological follow-up, all kidney tissue samples taken and control groups were analyzed with Hematoxylin-Eosin staining, and the nephrotoxic effect was immunoshistochemically with TGF-β and NF-κB and analyzed with a light microscope image analysis system. Results: Mussel-fed rats were found to cause inflammation in the kidney tissue, dilatation in the distal and proximal tubules, shrinkage in the glomeruli and degeneration in the tubular epithelium. In immunohistochemical staining, TGF-β and NF-κB immunoreactivity increased due to cells leading to inflammation and apoptosis. The nephrotoxic effect was quite high especially in rats given daily mussels. Conclusion: The mussel provided in unsanitary conditions may cause damage in kidney tissue from the excretory system organs due to excess consumption.

Keywords

• midye
• histopatoloji
• i̇mmünohistokimya
• böbrek
• mussel
• immunohistochemistry
• kidney
• histophatology
• nephrotoxicity