Enthesis Healing Is Dependent on Scaffold Interphase Morphology—Results from a Rodent Patellar Model
Carlos J. Peniche Silva,
Sebastian A. Müller,
Nicholas Quirk,
Patrina S. P. Poh,
Carla Mayer,
Antonella Motta,
Claudio Migliaresi,
Michael J. Coenen,
Christopher H. Evans,
Elizabeth R. Balmayor,
Martijn van Griensven
Affiliations
Carlos J. Peniche Silva
cBITE, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6229 ER Maastricht, The Netherlands
Sebastian A. Müller
Musculoskeletal Gene Therapy Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55905, USA
Nicholas Quirk
Musculoskeletal Gene Therapy Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55905, USA
Patrina S. P. Poh
Berlin Institute of Health at Charité–Universitätsmedizin Berlin, Julius Wolff Institute, 13353 Berlin, Germany
Carla Mayer
Department of General, Visceral and Minimally Invasive Surgery and Endoscopy, Dr. Lubos Kliniken Bogenhausen, 81679 Munich, Germany
Antonella Motta
BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, 38123 Trento, Italy
Claudio Migliaresi
BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, 38123 Trento, Italy
Michael J. Coenen
Musculoskeletal Gene Therapy Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55905, USA
Christopher H. Evans
Musculoskeletal Gene Therapy Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55905, USA
Elizabeth R. Balmayor
Musculoskeletal Gene Therapy Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55905, USA
Martijn van Griensven
cBITE, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6229 ER Maastricht, The Netherlands
The use of multiphasic scaffolds to treat injured tendon-to-bone entheses has shown promising results in vitro. Here, we used two versions of a biphasic silk fibroin scaffold to treat an enthesis defect created in a rat patellar model in vivo. One version presented a mixed transition between the bony and the tendon end of the construct (S-MT) while this transition was abrupt in the second version (S-AT). At 12 weeks after surgery, the S-MT scaffold promoted better healing of the injured enthesis, with minimal undesired ossification of the insertion area. The expression of tenogenic and chondrogenic markers was sustained for longer in the S-MT-treated group and the tangent modulus of the S-MT-treated samples was similar to the native tissue at 12 weeks while that of the S-AT-treated enthesis was lower. Our study highlights the important role of the transition zone of multiphasic scaffolds in the treatment of complex interphase tissues such as the tendon-to-bone enthesis.
