STAR Protocols (Jun 2023)
Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
- Amanda Labora,
- Hailey Lee,
- Charlotte Chan,
- Erin Tabornal,
- Thuc Le,
- Khalid Rashid,
- Evan Abt,
- Takanobu Yamao,
- Hanna Mandl,
- Amanda Creech,
- Alykhan Premji,
- Luyi Li,
- Jason Link,
- Nanping Wu,
- Caius Radu,
- Timothy Donahue
Affiliations
- Amanda Labora
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
- Hailey Lee
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Charlotte Chan
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Erin Tabornal
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Thuc Le
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Khalid Rashid
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Evan Abt
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Takanobu Yamao
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Hanna Mandl
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Amanda Creech
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Alykhan Premji
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Luyi Li
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Jason Link
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Nanping Wu
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Caius Radu
- Department of Molecular and Medical Pharmacology, Ahmanson Translational Imaging Division, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Timothy Donahue
- Department of Surgery, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
- Journal volume & issue
-
Vol. 4,
no. 2
p. 102163
Abstract
Summary: Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
