BMC Research Notes (Mar 2019)

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

  • Marcel Fernando da Silva,
  • Marcos Cesar Gonçalves,
  • Michael dos Santos Brito,
  • Paula Macedo Nóbile,
  • Larissa Mara de Andrade,
  • Cibele Nataliane Medeiros,
  • Silvana Creste,
  • Luciana Rossini Pinto

DOI
https://doi.org/10.1186/s13104-019-4168-5
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 7

Abstract

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Abstract Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls. Results The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algorithm and the Pearson correlation coefficients with the BestKeeper index. On the other hand, ribosomal protein L35-4 (RPL1), Actin (ACT) and Ubiquitin1 (UBQ1) were the least stable genes for all algorithms tested.

Keywords