Influence of Chlorpyrifos Stress on Protein Metabolism of Edible Crab Barytelphusa guerini, and its Recovery

Abstract

The sublethal stress of chlorpyrifos on important metabolites and enzymes of protein metabolism was investigated in most important tissues (gills, muscle, hepatopancreas and nervous tissue) of freshwater edible crab, Barytelphusa guerini. The crabs were exposed to 0.07 mg/l (1/3 of LC50) for 7, 14, 21 and 28 days. Subsequently, crabs were released into fresh water and kept in the same for 18 days in order to recovery study (3 days interval). Total protein content decreased whereas amino acids and ammonia increased in CPF exposed and maximum recovery was shown in cessation of intoxication. After cessation of intoxication recovery was observed. Urea content was decrease in all tissues and glutamine exhibited mixed response and recovery was highest in muscle. The activities of protease and aminotransferases were elevated in tissues for 28 days. Recovery of these enzymes activities was noticed during depuration. Acid phosphatase activity was inhibited in hepatopancreas and nervous ganglion and induced in gills and muscle. Alkaline phosphatase activity was enhanced in gills and hepatopancreas and inhibited in muscle and nervous ganglion. All these enzymes showed recovery after released of crabs into fresh water.

Keywords

• Barytelphusa guerini
• Chlorpyrifos
• Enzymes
• Protein