Transcriptome Profiling Reveals Candidate Key Genes Involved in Sinigrin Biosynthesis in <i>Brassica nigra</i>
Yang Li,
Youjian Yu,
Liai Xu,
Erbiao Guo,
Yunxiang Zang,
Yong He,
Zhujun Zhu
Affiliations
Yang Li
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Youjian Yu
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Liai Xu
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Erbiao Guo
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Yunxiang Zang
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Yong He
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Zhujun Zhu
Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Wusu Street 666, Lin’an, Hangzhou 311300, China
Glucosinolates (GSLs) are important secondary metabolites in Brassicales related to insect and disease resistance, flavor formation, and human health. Here, we determined the GSL profile with sinigrin as the predominant GSL in Brassica nigra. A total of 184 GSL biosynthetic genes (BniGSLs) were identified in B. nigra by a genome-wide search for orthologs of 82 of the 95 known GSL genes in Arabidopsis thaliana. Transcriptome data demonstrated that at least one BniGSL was highly expressed in stems and leaves at each step of the sinigrin synthesis pathway, which ensured the synthesis of a large amount of sinigrin in B. nigra. Among these key candidates of BniGSLs, the high expression of BniMAM1-2, BniCYP79F1, and BniAOP2-1/2, and the absence of MAM3 and AOP3, may contribute remarkably to the synthesis and accumulation of sinigrin. In addition, the low expression of some key BniGSLs partially explains the low content of indolic and aromatic GSLs in B. nigra. This study provided a genetic explanation for the formation of the unique GSL profile with sinigrin as the main GSL in B. nigra. The results of this study will be valuable for further functional analysis of BniGSLs and genetic improvement of GSLs in B. nigra and other Brassica species.
