Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges

Changsong Yang; Patricia Colosi; Siewert Hugelier; Daniel Zabezhinsky; Melike Lakadamyali; Tatyana Svitkina

doi:10.1038/s41467-022-33852-2

Nature Communications (Oct 2022)

Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges

Changsong Yang,
Patricia Colosi,
Siewert Hugelier,
Daniel Zabezhinsky,
Melike Lakadamyali,
Tatyana Svitkina

Affiliations

Changsong Yang: Department of Biology, University of Pennsylvania
Patricia Colosi: Department of Physiology, Perelman School of Medicine, University of Pennsylvania
Siewert Hugelier: Department of Physiology, Perelman School of Medicine, University of Pennsylvania
Daniel Zabezhinsky: Department of Biology, University of Pennsylvania
Melike Lakadamyali: Department of Physiology, Perelman School of Medicine, University of Pennsylvania
Tatyana Svitkina: Department of Biology, University of Pennsylvania

DOI: https://doi.org/10.1038/s41467-022-33852-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 20

Abstract

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The role of actin filaments in clathrin-mediated endocytosis (CME) is unclear. Here, Yang et al. show that branched actin filaments promote CME by pushing on clathrin coat edges in an epsin-dependent manner, dividing large flat clathrin plaques into sizes that facilitate invagination.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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