Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

Takashi Kanamoto; Takashi Tachibana; Yasushi Kitaoka; Toshio Hisatomi; Yasuhiro Ikeda; Yusuke Murakami; Kei Tobiume; Ryo Asaoka; Yoshiaki Kiuchi

doi:10.1155/2019/2431481

Journal of Ophthalmology (Jan 2019)

Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

Takashi Kanamoto,
Takashi Tachibana,
Yasushi Kitaoka,
Toshio Hisatomi,
Yasuhiro Ikeda,
Yusuke Murakami,
Kei Tobiume,
Ryo Asaoka,
Yoshiaki Kiuchi

Affiliations

Takashi Kanamoto: Department of Ophthalmology, Hiroshima Memorial Hospital, 1-4-3, Honkawacho, Naka-ku, Hiroshima 730-0802, Japan
Takashi Tachibana: Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Yasushi Kitaoka: Department of Ophthalmology, St. Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki City, Kanagawa 216-8511, Japan
Toshio Hisatomi: Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Yasuhiro Ikeda: Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Yusuke Murakami: Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Kei Tobiume: Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan
Ryo Asaoka: Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
Yoshiaki Kiuchi: Department of Ophthalmology and Visual Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan

DOI: https://doi.org/10.1155/2019/2431481
Journal volume & issue: Vol. 2019

Abstract

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Purpose. To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods. Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results. D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion. Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.

Published in Journal of Ophthalmology

ISSN: 2090-004X (Print); 2090-0058 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Ophthalmology
Website: https://onlinelibrary.wiley.com/journal/3846

About the journal