Group 2 Innate Lymphoid Cells Exacerbate Amebic Liver Abscess in Mice
Risa Nakamura,
Akihiro Yoshizawa,
Taeko Moriyasu,
Sharmina Deloer,
Masachika Senba,
Mihoko Kikuchi,
Shigeo Koyasu,
Kazuyo Moro,
Shinjiro Hamano
Affiliations
Risa Nakamura
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Graduate School of Biomedical Sciences Doctoral Leadership Program, Nagasaki University, Nagasaki, Japan; The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Corresponding author
Akihiro Yoshizawa
Laboratory for Innate Immune Systems, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Japan; Department of Cardiovascular Medicine, International University of Health and Welfare (IUHW), School of Medicine, Chiba, Japan
Taeko Moriyasu
Graduate School of Biomedical Sciences Doctoral Leadership Program, Nagasaki University, Nagasaki, Japan; The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Kenya Research Station, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan
Sharmina Deloer
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Graduate School of Biomedical Sciences Doctoral Leadership Program, Nagasaki University, Nagasaki, Japan; The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Mucosal Immunity Section, Laboratory of Clinical Immunology and Microbiology (LCIM), National Institute of Allergy and infectious Diseases (NIAID), NIH, Maryland, USA
Masachika Senba
The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Department of Pathology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan
Mihoko Kikuchi
The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan
Shigeo Koyasu
Laboratory for Immune Cell Systems, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan
Kazuyo Moro
Laboratory for Innate Immune Systems, RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, Japan; Laboratory for Innate Immune Systems, Osaka University Graduate School of Medicine, Osaka, Japan
Shinjiro Hamano
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Graduate School of Biomedical Sciences Doctoral Leadership Program, Nagasaki University, Nagasaki, Japan; The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan; Corresponding author
Summary: Entamoeba histolytica, a protozoan parasite in the lumen of the human large intestine, occasionally spreads to the liver and induces amebic liver abscesses (ALAs). Upon infection with E. histolytica, high levels of type 2 cytokines are induced in the liver early after infection. However, the sources and functions of these initial type 2 cytokines in ALA formation remain unclear. In this study, we examined the roles of group 2 innate lymphoid cells (ILC2s) in ALA formation. Hepatic ILC2 numbers were significantly increased and they produced robust levels of IL-5. The in vivo transfer of ILC2s into Rag2−/−common γ chain (γc)−/− KO mice aggravated ALA formation accompanied by eosinophilia and neutrophilia. Furthermore, IL-33-deficient mice and IL-5-neutralized mice had less ALA formations. These results suggest that ILC2s contribute to exacerbating the pathogenesis of ALA by producing early type 2 cytokines and promoting the accumulation of eosinophils and neutrophils in the liver.
