ХОЁР БӨХТ ТЭМЭЭ (CAMELUS BACTRIANUS)-НИЙ БУУРЫН БОХИНЫ УУРГИЙН IN VITRO ДАХЬ ХҮНИЙ ХАВДРЫН БОЛОН ХАВДРЫН БУС ЭСИЙН ҮЙЛ АЖИЛЛАГААНД ҮЗҮҮЛЭХ НӨЛӨӨГ СУДАЛСАН ДҮНГЭЭС

Abstract

Various natural products derived from plants and animals have being played important role in the treatment of human diseases for many centuries. One of those products is poll gland secretion of camel (Camelus bactrianus). During rutting season poll gland of male camel becomes bigger and produces secretion. In traditional medicine, poll gland secretion is used in cancer treatment especially breast cancer, anti-inflammation, softening gall-stone and some other treatments. In this study, we isolated total protein from poll gland of camel, and detected antioxidant activity and studied effects of secretion protein in vitro cancer and non-cancer cell lines. Antioxidant activity was correlated with their protein level. The half maximal inhibitory concentration of cancer cell (IC50) was identified differently in cell lines, IC50 of HCC1397 breast cancer cell line was at 407µg/ml concentration of protein whereas IC50 of HepG2 liver cancer cell line was at 215µg/ml. The protein had effect in cancer cell mobility by suppressing 80% to 100% when the protein concentration was 200µg/ml. Our result showed that the poll gland protein has negative effects to some genes such as CASP3 and CASP8 which are involved in cell apoptosis.

Keywords

• poll gland secretion, cancer, cell line, apoptosis, gene expression