Nature Communications (May 2019)

Switching the activity of Cas12a using guide RNA strand displacement circuits

  • Lukas Oesinghaus,
  • Friedrich C. Simmel

DOI
https://doi.org/10.1038/s41467-019-09953-w
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Cas12a is a useful alternative to Cas9 for genome editing and regulation. Here the authors design strand displacement gRNAs that can add functionality to Cas12a by acting as multi-input logic gates.