A vaccinology Approach to the Identification and Characterization of <i>Dermanyssus Gallinae</i> Candidate Protective Antigens for the Control of Poultry Red Mite Infestations
José Francisco Lima-Barbero,
Marinela Contreras,
Lourdes Mateos-Hernández,
Francisco Manuel Mata-Lorenzo,
Roxana Triguero-Ocaña,
Olivier Sparagano,
Robert D. Finn,
Christina Strube,
Daniel R.G. Price,
Francesca Nunn,
Kathryn Bartley,
Ursula Höfle,
Mariana Boadella,
Alasdair J. Nisbet,
José de la Fuente,
Margarita Villar
Affiliations
José Francisco Lima-Barbero
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Marinela Contreras
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Lourdes Mateos-Hernández
UMR BIPAR, INRA, Ecole Nationale Vétérinaire d´Alfort, ANSES, Université Paris-Est, 94700 Maisons-Alfort, France
Francisco Manuel Mata-Lorenzo
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Roxana Triguero-Ocaña
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Olivier Sparagano
Department of Infectious Diseases and Public Health, City University of Hong Kong, Kowloon, Hong Kong SAR
Robert D. Finn
Department of Applied Sciences, Faculty of Health & Life Sciences, Northumbria University, Newcastle Upon Tyne, NE1 8ST, UK
Christina Strube
Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
Daniel R.G. Price
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK
Francesca Nunn
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK
Kathryn Bartley
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK
Ursula Höfle
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Mariana Boadella
Sabiotec, S.A. Ed. Polivalente UCLM. Camino de Moledores. 13005. Ciudad Real, Spain
Alasdair J. Nisbet
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK
José de la Fuente
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
Margarita Villar
SaBio. Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071, Ciudad Real, Spain
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. Rhipicephalus microplus Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control.
