Sargachromenol Purified from <i>Sargassum horneri</i> Inhibits Inflammatory Responses via Activation of Nrf2/HO-1 Signaling in LPS-Stimulated Macrophages
Eui-Jeong Han,
Thilina U. Jayawardena,
Jae-Hyuk Jang,
Ilekuttige Priyan Shanura Fernando,
Youngheun Jee,
You-Jin Jeon,
Dae-Sung Lee,
Jeong-Min Lee,
Mi-Jin Yim,
Lei Wang,
Hyun-Soo Kim,
Ginnae Ahn
Affiliations
Eui-Jeong Han
Research Center for Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
Thilina U. Jayawardena
Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
Jae-Hyuk Jang
Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea
Ilekuttige Priyan Shanura Fernando
Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea
Youngheun Jee
Department of Veterinary Medicine, Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea
You-Jin Jeon
Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
Dae-Sung Lee
National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea
Jeong-Min Lee
National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea
Mi-Jin Yim
National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea
Lei Wang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
Hyun-Soo Kim
National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea
Ginnae Ahn
Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea
In this study, we isolated sargachromenol (SC) from Sargassum horneri and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. SC did not show cytotoxicity at all concentrations and effectively increased the cell viability by reducing the nitric oxide (NO) and intracellular reactive oxygen species (ROS) production in LPS-stimulated RAW 264.7 macrophages. In addition, SC decreased the mRNA expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and inflammatory mediators (iNOS and COX-2). Moreover, SC suppressed the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) and mitogen-activated protein kinase (MAPK) signaling, whereas activated the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling in LPS-stimulated RAW 264.7 macrophages. Interestingly, the anti-inflammatory effect of SC was abolished by the inhibition of HO-1 in LPS-stimulated RAW 264.7 macrophages. According to the results, this study suggests that the antioxidant capacity of SC leads to its anti-inflammatory effect and it potentially may be utilized in the nutraceutical and pharmaceutical sectors.
