Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9

Jinbing Li; Yuhang Li; Xiao-Yong Zhan; Xinru Ran; Hung-Fat Tse; Shiying Dang; Yichu Nie; Ke Huang

Stem Cell Research (Dec 2020)

Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9

Jinbing Li,
Yuhang Li,
Xiao-Yong Zhan,
Xinru Ran,
Hung-Fat Tse,
Shiying Dang,
Yichu Nie,
Ke Huang

Affiliations

Jinbing Li: University of Chinese Academy of Sciences, Beijing 100049, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
Yuhang Li: CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
Xiao-Yong Zhan: The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China
Xinru Ran: Cardiology Division, Department of Medicine, The University of Hong Kong, Hong Kong SAR, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, China
Hung-Fat Tse: Cardiology Division, Department of Medicine, The University of Hong Kong, Hong Kong SAR, China
Shiying Dang: The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China
Yichu Nie: Department of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, Shenzhen 518107, China; Corresponding authors at: The Seventh Affiliated Hospital, Sun Yat-sen University, 628 Zhenyuan Road, Guangming Street, Guangming New District, Shenzhen 518107, China (K. Huang). Department of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, 66 Gongchang Road, Guangming Street, Guangming New District, Shenzhen 518107, China (Y. Nie).
Ke Huang: The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Corresponding authors at: The Seventh Affiliated Hospital, Sun Yat-sen University, 628 Zhenyuan Road, Guangming Street, Guangming New District, Shenzhen 518107, China (K. Huang). Department of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, 66 Gongchang Road, Guangming Street, Guangming New District, Shenzhen 518107, China (Y. Nie).

Journal volume & issue: Vol. 49
p. 102090

Abstract

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GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been merely documented. To illustrate the function of GADD45A in the human embryonic stem cell biology, we reported a GADD45A knockout human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting. This cell line displayed normal karyotype, pluripotent stem cell marker expression and differentiation potential both in vivo and vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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