A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion

Sam J. B. Mallinson; Melodie M. Machovina; Rodrigo L. Silveira; Marc Garcia-Borràs; Nathan Gallup; Christopher W. Johnson; Mark D. Allen; Munir S. Skaf; Michael F. Crowley; Ellen L. Neidle; Kendall N. Houk; Gregg T. Beckham; Jennifer L. DuBois; John E. McGeehan

doi:10.1038/s41467-018-04878-2

Nature Communications (Jun 2018)

A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion

Sam J. B. Mallinson,
Melodie M. Machovina,
Rodrigo L. Silveira,
Marc Garcia-Borràs,
Nathan Gallup,
Christopher W. Johnson,
Mark D. Allen,
Munir S. Skaf,
Michael F. Crowley,
Ellen L. Neidle,
Kendall N. Houk,
Gregg T. Beckham,
Jennifer L. DuBois,
John E. McGeehan

Affiliations

Sam J. B. Mallinson: Molecular Biophysics, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth
Melodie M. Machovina: National Bioenergy Center, National Renewable Energy Laboratory
Rodrigo L. Silveira: National Bioenergy Center, National Renewable Energy Laboratory
Marc Garcia-Borràs: Department of Chemistry and Biochemistry, University of California at Los Angeles
Nathan Gallup: National Bioenergy Center, National Renewable Energy Laboratory
Christopher W. Johnson: National Bioenergy Center, National Renewable Energy Laboratory
Mark D. Allen: Molecular Biophysics, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth
Munir S. Skaf: Institute of Chemistry, University of Campinas
Michael F. Crowley: Biosciences Center, National Renewable Energy Laboratory
Ellen L. Neidle: Department of Microbiology, University of Georgia
Kendall N. Houk: Department of Chemistry and Biochemistry, University of California at Los Angeles
Gregg T. Beckham: National Bioenergy Center, National Renewable Energy Laboratory
Jennifer L. DuBois: Department of Chemistry and Biochemistry, Montana State University
John E. McGeehan: Molecular Biophysics, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth

DOI: https://doi.org/10.1038/s41467-018-04878-2
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Catabolizing lignin-derived aromatic compounds requires an aryl-O-demethylation step. Here the authors present the structures of GcoA and GcoB, a cytochrome P450-reductase pair that catalyzes aryl-O-demethylations and show that GcoA displays broad substrate specificity, which is of interest for biotechnology applications.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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