The proliferating cell nuclear antigen gene (pcna) plays a key role in ovarian development in the ridgetail white prawn, Exopalaemon carinicauda

Songsong Hua; Wanying Li; Duwei Zheng; Xinyu Zhou; Sichen Zhang; Huimin Zhang; Xue Liu; Wazir Ali Baloch; Binlun Yan; Huan Gao

Aquaculture Reports (Jun 2024)

The proliferating cell nuclear antigen gene (pcna) plays a key role in ovarian development in the ridgetail white prawn, Exopalaemon carinicauda

Songsong Hua,
Wanying Li,
Duwei Zheng,
Xinyu Zhou,
Sichen Zhang,
Huimin Zhang,
Xue Liu,
Wazir Ali Baloch,
Binlun Yan,
Huan Gao

Affiliations

Songsong Hua: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Wanying Li: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Duwei Zheng: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China
Xinyu Zhou: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Sichen Zhang: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Huimin Zhang: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Xue Liu: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Wazir Ali Baloch: Department of Freshwater Biology and Fisheries, University of Sindh Jamshoro, Sindh 76080, Pakistan
Binlun Yan: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China
Huan Gao: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, Jiangsu 222005, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, Jiangsu 210014, China; Corresponding author at: Jiangsu Key Laboratory of Marine Bioresources and Environment / Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China.

Journal volume & issue: Vol. 36
p. 102170

Abstract

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The ridgetail white prawn, Exopalaemon carinicauda, has advantages in its rapid growth and strong environmental adaptability, and it is a good species for the biological research of crustaceans. To explore the function of the pcna gene in the ovarian development of crustaceans, we cloned the pcna (Ec-pcna) from E. carinicauda. The open reading frame of the pcna is 786 bp, and it encodes a total of 261 aa. A PCNA/RFC (replication factor C) domain exists at 208–254 aa, and according to a phylogenetic analysis, the amino acid sequence of Ec-pcna has the highest similarity with the pcna gene from Litopenaeus vannamei, and it is clustered into one branch with L. vannamei and Penaeus monodon. The results of qRT-PCR showed that the expression of pcna was highest in the ovarian tissue of E. carinicauda, and it was significantly higher than that of other tissues (p<0.05). In addition, the expression level gradually increased in Stages I-III of ovarian development, with the expression level in Stage III being significantly higher compared to the other stages (p<0.05). The protein overexpression analysis showed that the overexpression of the PCNA protein was due to an overexpression of cell cycle-related genes cdk2 and cyclin A, and accelerated ovarian development. Immunohistochemical results showed that pcna played an important role in the accumulation of exogenous nutrients. Moreover, the RNAi results showed that knocking down the expression of the pcna gene can significantly reduce the expression of the gene Vg, related to vitelline, indicating that pcna has a regulatory effect on the expression of Vg. Based on the results of flow cytometry, we speculate that the decreasing of vg expression level in the ovary may be caused by the inhibition of follicular cell polyploidization.This study showed that pcna plays an important role in the proliferation of ovarian cells and the accumulation of exogenous nutrients in E. carinicauda.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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