Toxins (Jan 2020)

Role of Shiga Toxins in Cytotoxicity and Immunomodulatory Effects of <i>Escherichia coli</i> O157:H7 during Host-Bacterial Interactions in vitro

  • Andrea Cecilia Bruballa,
  • Carolina Maiumi Shiromizu,
  • Alan Mauro Bernal,
  • Gonzalo Ezequiel Pineda,
  • Florencia Sabbione,
  • Analia Silvina Trevani,
  • Leticia Verónica Bentancor,
  • María Victoria Ramos,
  • Romina Jimena Fernández-Brando,
  • Manuel Javier Muñoz,
  • Marina Sandra Palermo

DOI
https://doi.org/10.3390/toxins12010048
Journal volume & issue
Vol. 12, no. 1
p. 48

Abstract

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Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx2 transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1β and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx2-specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections.

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