Revista da Sociedade Brasileira de Medicina Tropical (Oct 2015)

Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

  • Francesca Guaracyaba Garcia Chapadense,
  • Everton Kort Kamp Fernandes,
  • José Bento Pereira Lima,
  • Ademir Jesus Martins,
  • Luana Carrara Silva,
  • Welington Tristão da Rocha,
  • Adelair Helena dos Santos,
  • Pedro Cravo

DOI
https://doi.org/10.1590/0037-8682-0046-2015
Journal volume & issue
Vol. 48, no. 5
pp. 607 – 609

Abstract

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ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.

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