Communications Biology (Jan 2023)
A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
- Chia-Feng Tsai,
- Yi-Ting Wang,
- Chuan-Chih Hsu,
- Reta Birhanu Kitata,
- Rosalie K. Chu,
- Marija Velickovic,
- Rui Zhao,
- Sarah M. Williams,
- William B. Chrisler,
- Marda L. Jorgensen,
- Ronald J. Moore,
- Ying Zhu,
- Karin D. Rodland,
- Richard D. Smith,
- Clive H. Wasserfall,
- Tujin Shi,
- Tao Liu
Affiliations
- Chia-Feng Tsai
- Biological Sciences Division, Pacific Northwest National Laboratory
- Yi-Ting Wang
- Biological Sciences Division, Pacific Northwest National Laboratory
- Chuan-Chih Hsu
- Institute of Plant and Microbial Biology, Academia Sinica
- Reta Birhanu Kitata
- Biological Sciences Division, Pacific Northwest National Laboratory
- Rosalie K. Chu
- Biological Sciences Division, Pacific Northwest National Laboratory
- Marija Velickovic
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Rui Zhao
- Biological Sciences Division, Pacific Northwest National Laboratory
- Sarah M. Williams
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- William B. Chrisler
- Biological Sciences Division, Pacific Northwest National Laboratory
- Marda L. Jorgensen
- Department of Pathology, Immunology, and Laboratory Medicine, Diabetes Institute, College of Medicine, University of Florida
- Ronald J. Moore
- Biological Sciences Division, Pacific Northwest National Laboratory
- Ying Zhu
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Karin D. Rodland
- Biological Sciences Division, Pacific Northwest National Laboratory
- Richard D. Smith
- Biological Sciences Division, Pacific Northwest National Laboratory
- Clive H. Wasserfall
- Department of Pathology, Immunology, and Laboratory Medicine, Diabetes Institute, College of Medicine, University of Florida
- Tujin Shi
- Biological Sciences Division, Pacific Northwest National Laboratory
- Tao Liu
- Biological Sciences Division, Pacific Northwest National Laboratory
- DOI
- https://doi.org/10.1038/s42003-022-04400-x
- Journal volume & issue
-
Vol. 6,
no. 1
pp. 1 – 12
Abstract
A streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics is developed, reducing sample loss and processing time, allowing the phosphoproteome profiling of mass-limited samples at the low nanogram level.
