Regenerative Therapy (Mar 2024)
Fabrication of novel nanofiber composed of gelatin/alginate with zirconium oxide NPs regulate orthodontic progression of cartilage degeneration on Wnt/β-catenin signaling axis in MC3T3-E1 cells
Abstract
Natural macromolecules like alginate and gelatin are employed to create medication delivery systems that are both safe and effective. Zirconium nanoparticles (ZrO2 NPs) have been proposed as a means of enhancing the alginate-gelatin hydrogel's physical and biological properties. This study combines the synthesis of the biopolymers gelatin and alginate nanofibers with nanoparticles of zirconium oxide (GA/NF– ZrO2 NPs). UV, XRD, FTIR, and SEM were used to characterize the synthesized nanofibers. The expression of osteogenic genes was analyzed by western blotting and qualitative real-time polymerase chain reaction (qRT-PCR). Based on our findings, MC3T3-E1 cells are performed for cell viability, apoptosis and reactive oxygen species production by GA/NF– ZrO2 NPs through the Wnt/β-catenin signaling pathway. Cell migration was accelerated at 75 μg/mL concentration after 24 h of damage in a scratch wound healing assay. Proliferation of the MC3T3-E1 cell line was also detected. GA/NF–ZrO2 NPs influenced the osteogenic variation of MC3T3-E1 cells by inducing autophagy. Furthermore, the impact of obstruction on the temporomandibular joint (TMJ) is a subject of ongoing discussion and analysis within the context of animal models. Coordinated GA/NF–ZrO2 NPs on biomaterial nanofibers could be used to introduce physical signals for modifying MC3T3-E1 responds for orthodontic engineering constructs.
