LC-MS-MS identification of drug metabolites obtained by metalloporphyrin mediated oxidation

Abstract

In this paper we report the application of liquid chromatography-mass spectrometry (LC-MS-MS) to the identification of the products formed by oxidation of albendazole and disopyramide with metalloporphyrins in dichloroethane, using iodosylbenzene as an oxygen donor. Our results show that LC-MS-MS is a powerful tool to study the in vitro metabolism of drugs, allowing the identification of known and unknown metabolites. In addition, it was observed that the catalyst system used resulted in the formation of the same metabolites as obtained in vivo, although for disopyramide other products were also observed.

Keywords

• LC-MS-MS
• metabolism
• metalloporphyrin
• disopyramide
• albendazole