ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL

Abstract

Histological and microbiological comparative analysis of bovine pericardium preserved for 30 and 60 days in glycerin (98%), supersaturated sugar solution (300%), supersaturated solution of salt (150%) and unprocessed honey were performed aiming to establish alternative ways of preservation of biological membranes. Samples of pericardium from five cattle were collected in fridge and maintained for up to 60 days in containers with the above mentioned solutions of conservation. For morphological analysis of the constituents of bovine pericardium were performed routine histological techniques associated to two microbiological culture techniques: streaking on the surface of the plate and pour-plate. The supersaturated solution of sugar (300%) was the medium of preservation with results closer to the obtained with glycerin (98%), but was unable to prevent microorganisms growth. The unprocessed honey and supersaturated solution of salt (150%) had suitable antiseptic characteristics, but failed to effectively conserve the pericardium, with a greater structural disorganization of the connective tissue, indicating advanced process of autolysis after 60 days of storage. It was concluded that glycerin (98%) appeared as the most efficient solution when compared to the others evaluated in this study.

Keywords

• Preservation methods
• Pericardium
• Biological membrane