<i>Lactobacillus gasseri</i> LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
Mariana de Moura e Dias,
Vinícius da Silva Duarte,
Lúcio Flávio Macedo Mota,
Gabriela de Cássia Ávila Alpino,
Sandra Aparecida dos Reis Louzano,
Lisiane Lopes da Conceição,
Hilário Cuquetto Mantovanie,
Solange Silveira Pereira,
Leandro Licursi Oliveira,
Tiago Antônio de Oliveira Mendes,
Davide Porcellato,
Maria do Carmo Gouveia Peluzio
Affiliations
Mariana de Moura e Dias
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Vinícius da Silva Duarte
Faculty of Chemistry, Biotechnology, and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway
Lúcio Flávio Macedo Mota
Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell’Università, 16, 35020 Padua, Italy
Gabriela de Cássia Ávila Alpino
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Sandra Aparecida dos Reis Louzano
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Lisiane Lopes da Conceição
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Hilário Cuquetto Mantovanie
Department of Microbiology, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Solange Silveira Pereira
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Leandro Licursi Oliveira
Department of General Biology, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Tiago Antônio de Oliveira Mendes
Department of Biochemistry and Molecular Biology, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Davide Porcellato
Faculty of Chemistry, Biotechnology, and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway
Maria do Carmo Gouveia Peluzio
Department of Nutrition and Health, Universidade Federal de Vicosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa 36570-900, Minas Gerais, Brazil
Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and improving intestinal health. Accordingly, obesity was induced in C57BL/6J mice, after which they were redistributed and fed with an obesogenic diet (intervention A) or standard AIN-93 diet (intervention B). Concomitantly, all the groups underwent a treatment phase with Lactobacillus gasseri LG-G12, ceftriaxone, or ceftriaxone followed by L. gasseri LG-G12. At the end of the experimental period, the following analysis was conducted: metataxonomic analysis, functional profiling of gut microbiota, intestinal permeability, and caecal concentration of short-chain fatty acids. High-fat diet impaired bacterial diversity/richness, which was counteracted in association with L. gasseri LG-G12 and the AIN-93 diet. Additionally, SCFA-producing bacteria were negatively correlated with high intestinal permeability parameters, which was further confirmed via functional profile prediction of the gut microbiota. A novel perspective on anti-obesity probiotics is presented by these findings based on the improvement of intestinal health irrespective of undergoing antimicrobial therapy or not.
