Influence of Dietary Inulin on Fecal Microbiota, Cardiometabolic Risk Factors, Eicosanoids, and Oxidative Stress in Rats Fed a High-Fat Diet
Bernat Miralles-Pérez,
Maria Rosa Nogués,
Vanessa Sánchez-Martos,
Àngels Fortuño-Mar,
Sara Ramos-Romero,
Josep L. Torres,
Julia Ponomarenko,
Susana Amézqueta,
Xiang Zhang,
Marta Romeu
Affiliations
Bernat Miralles-Pérez
Functional Nutrition, Oxidation and Cardiovascular Diseases Research Group (NFOC-SALUT), Pharmacology Unit, Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/ Sant Llorenç 21, E-43201 Reus, Spain
Maria Rosa Nogués
Functional Nutrition, Oxidation and Cardiovascular Diseases Research Group (NFOC-SALUT), Pharmacology Unit, Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/ Sant Llorenç 21, E-43201 Reus, Spain
Vanessa Sánchez-Martos
Functional Nutrition, Oxidation and Cardiovascular Diseases Research Group (NFOC-SALUT), Pharmacology Unit, Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/ Sant Llorenç 21, E-43201 Reus, Spain
Department of Biological Chemistry, Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), C/ Jordi Girona 18–26, E-08034 Barcelona, Spain
Josep L. Torres
Department of Biological Chemistry, Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), C/ Jordi Girona 18–26, E-08034 Barcelona, Spain
Julia Ponomarenko
Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Universitat Pompeu Fabra (UPF), E-08003 Barcelona, Spain
Susana Amézqueta
Departament d’Enginyeria Química i Química Analítica and Institut de Biomedicina (IBUB), Universitat de Barcelona, E-08028 Barcelona, Spain
Xiang Zhang
Department of Chemistry, University of Louisville, 2210 S. Brook Street, Louisville, KY E-40292, USA
Marta Romeu
Functional Nutrition, Oxidation and Cardiovascular Diseases Research Group (NFOC-SALUT), Pharmacology Unit, Department of Basic Medical Sciences, Universitat Rovira i Virgili, C/ Sant Llorenç 21, E-43201 Reus, Spain
The present study examined the influence of inulin on fecal microbiota, cardiometabolic risk factors, eicosanoids, and oxidative stress in rats on a high-fat (HF) diet. Thirty-six male Wistar–Kyoto rats were divided into three dietary groups: standard diet, HF diet, and HF diet + Inulin diet. After 10 weeks, the HF + Inulin diet promoted high dominance of a few bacterial genera including Blautia and Olsenella in feces while reducing richness, diversity, and rarity compared to the HF diet. These changes in fecal microbiota were accompanied by an increased amount of propionic acid in feces. The HF + Inulin diet decreased cardiometabolic risk factors, decreased the amount of the eicosanoids 11(12)-EET and 15-HETrE in the liver, and decreased oxidative stress in blood compared to the HF diet. In conclusion, increasing consumption of inulin may be a useful nutritional strategy to protect against the onset of obesity and its associated metabolic abnormalities by means of modulation of gut microbiota.