Journal of Synchrotron Radiation (May 2022)
Observations of phase changes in monoolein during high viscous injection
- Daniel J. Wells,
- Peter Berntsen,
- Eugeniu Balaur,
- Cameron M. Kewish,
- Patrick Adams,
- Andrew Aquila,
- Jack Binns,
- Sébastien Boutet,
- Hayden Broomhall,
- Carl Caleman,
- Andrew Christofferson,
- Charlotte E. Conn,
- Caroline Dahlqvist,
- Leonie Flueckiger,
- Francisco Gian Roque,
- Tamar L. Greaves,
- Majid Hejazian,
- Mark Hunter,
- Marjan Hadian Jazi,
- H. Olof Jönsson,
- Sachini Kadaoluwa Pathirannahalage,
- Richard A. Kirian,
- Alex Kozlov,
- Ruslan P. Kurta,
- Hugh Marman,
- Derek Mendez,
- Andrew Morgan,
- Keith Nugent,
- Dominik Oberthuer,
- Harry Quiney,
- Juliane Reinhardt,
- Saumitra Saha,
- Jonas A. Sellberg,
- Raymond Sierra,
- Max Wiedorn,
- Brian Abbey,
- Andrew V. Martin,
- Connie Darmanin
Affiliations
- Daniel J. Wells
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Peter Berntsen
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Eugeniu Balaur
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Cameron M. Kewish
- Australian Synchrotron, Australian Nuclear Science and Technology Organisation, 800 Blackburn Road, Clayton, VIC 3168, Australia
- Patrick Adams
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Andrew Aquila
- SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Jack Binns
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Sébastien Boutet
- SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Hayden Broomhall
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Carl Caleman
- Department of Physics and Astronomy, Uppsala University, Box 516, SE-751 20 Uppsala, Sweden
- Andrew Christofferson
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Charlotte E. Conn
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Caroline Dahlqvist
- Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden
- Leonie Flueckiger
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Francisco Gian Roque
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Tamar L. Greaves
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Majid Hejazian
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Mark Hunter
- SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Marjan Hadian Jazi
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- H. Olof Jönsson
- Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden
- Sachini Kadaoluwa Pathirannahalage
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Richard A. Kirian
- Department of Physics, Arizona State University, Tempe, AZ 85287, USA
- Alex Kozlov
- ARC Centre of Excellence for Advanced Molecular Imaging, The University of Melbourne, Parkville, VIC 3010, Australia
- Ruslan P. Kurta
- European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany
- Hugh Marman
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Derek Mendez
- Department of Physics, Arizona State University, Tempe, AZ 85287, USA
- Andrew Morgan
- ARC Centre of Excellence for Advanced Molecular Imaging, The University of Melbourne, Parkville, VIC 3010, Australia
- Keith Nugent
- Research School of Physics, The Australian National University, Acton, ACT, Australia
- Dominik Oberthuer
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Harry Quiney
- ARC Centre of Excellence for Advanced Molecular Imaging, The University of Melbourne, Parkville, VIC 3010, Australia
- Juliane Reinhardt
- Australian Synchrotron, Australian Nuclear Science and Technology Organisation, 800 Blackburn Road, Clayton, VIC 3168, Australia
- Saumitra Saha
- ARC Centre of Excellence for Advanced Molecular Imaging, The University of Melbourne, Parkville, VIC 3010, Australia
- Jonas A. Sellberg
- Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden
- Raymond Sierra
- SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Max Wiedorn
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany
- Brian Abbey
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- Andrew V. Martin
- School of Science, RMIT University, Melbourne, VIC 3000, Australia
- Connie Darmanin
- La Trobe Institute for Molecular Science, Department of Mathematical and Physical Sciences, School of Computing Engineering and Mathematical Science, La Trobe University, Bundoora, VIC 3086, Australia
- DOI
- https://doi.org/10.1107/S1600577522001862
- Journal volume & issue
-
Vol. 29,
no. 3
pp. 602 – 614
Abstract
Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam. Typically, the carrier medium is a lipidic cubic phase (LCP) media, which can also be used to nucleate and grow the crystals. However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not been reported and hence is not yet well understood. The self-assembled structure of the LCP can be affected by pressure, dehydration and temperature changes, all of which occur during continuous flow injection. These changes to the LCP structure may in turn impact the results of X-ray diffraction measurements from membrane protein crystals. To investigate the influence of HVIs on the structure of the LCP we conducted a study of the phase changes in monoolein/water and monoolein/buffer mixtures during continuous flow injection, at both atmospheric pressure and under vacuum. The reservoir pressure in the HVI was tracked to determine if there is any correlation with the phase behaviour of the LCP. The results indicated that, even though the reservoir pressure underwent (at times) significant variation, this did not appear to correlate with observed phase changes in the sample stream or correspond to shifts in the LCP lattice parameter. During vacuum injection, there was a three-way coexistence of the gyroid cubic phase, diamond cubic phase and lamellar phase. During injection at atmospheric pressure, the coexistence of a cubic phase and lamellar phase in the monoolein/water mixtures was also observed. The degree to which the lamellar phase is formed was found to be strongly dependent on the co-flowing gas conditions used to stabilize the LCP stream. A combination of laboratory-based optical polarization microscopy and simulation studies was used to investigate these observations.
Keywords
