3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds
Maria A. Khvorostina,
Anton V. Mironov,
Irina A. Nedorubova,
Tatiana B. Bukharova,
Andrey V. Vasilyev,
Dmitry V. Goldshtein,
Vladimir S. Komlev,
Vladimir K. Popov
Affiliations
Maria A. Khvorostina
Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, Russia
Anton V. Mironov
Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, Russia
Irina A. Nedorubova
Research Centre for Medical Genetics, Moscow 115478, Russia
Tatiana B. Bukharova
Research Centre for Medical Genetics, Moscow 115478, Russia
Andrey V. Vasilyev
Research Centre for Medical Genetics, Moscow 115478, Russia
Dmitry V. Goldshtein
Research Centre for Medical Genetics, Moscow 115478, Russia
Vladimir S. Komlev
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Moscow 119334, Russia
Vladimir K. Popov
Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, Russia
Gene therapy is one of the most promising approaches in regenerative medicine to restore damaged tissues of various types. However, the ability to control the dose of bioactive molecules in the injection site can be challenging. The combination of genetic constructs, bioresorbable material, and the 3D printing technique can help to overcome these difficulties and not only serve as a microenvironment for cell infiltration but also provide localized gene release in a more sustainable way to induce effective cell differentiation. Herein, the cell transfection with plasmid DNA directly incorporated into sodium alginate prior to 3D printing was investigated both in vitro and in vivo. The 3D cryoprinting ensures pDNA structure integrity and safety. 3D printed gene-activated scaffolds (GAS) mediated HEK293 transfection in vitro and effective synthesis of model EGFP protein in vivo, thereby allowing the implementation of the developed GAS in future tissue engineering applications.
