Influence of bacterial metabolites from permafrost on morphophysiological parameters of potato material in vitro*

Abstract

The effect of secondary metabolites of bacteria from permafrost rocks on the in vitro morphophysiological parameters of potato microplants has been studied. Microplants of Zhukovsky ranniy potato variety were used as the object of the study. The experimental results indicate that when potato microplants are grown in vitro together in the Murashige-Skoog nutrient medium with the bacteria metabolites of the Bacillus cereus 9-08-CH9 and Achromobacter spanius 10-50TS2 strains introduced at the grafting time at a dose of 250 μl, they have the highest inhibitory effect. Secondary metabolites of these bacterial strains have a significant increase in the number of internodes at all stages of plant cultivation, which can accelerate the in vitro replication of material for original potato seed production. Metabolites of the bacterial strain Bacillus cereus 875TS in the indicated concentrations cause growth inhibition and delay in the development of microplants’ root system at the initial cultivation stages.