Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
Camille Delude,
Sollapura Vishwanath,
Owen Rowland,
Frédéric Domergue
Affiliations
Camille Delude
Laboratoire de Biogenèse Membranaire, UMR 5200, CNRS – Université de Bordeaux, INRA Bordeaux Aquitaine, Villenave d’Ornon, France
Sollapura Vishwanath
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Government of Canada, Ottawa, CanadaDepartment of Biology and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Canada
Owen Rowland
Department of Biology and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Canada
Frédéric Domergue
Laboratoire de Biogenèse Membranaire, UMR 5200, CNRS – Université de Bordeaux, INRA Bordeaux Aquitaine, Villenave d’Ornon, France
Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) present in roots. For the solvent-extraction method, roots are first delipidated before transmethylation. This method is longer but allows separation of soluble and polymerized root lipids. This protocol is optimized for tissue culture- or soil-grown Arabidopsis thaliana plants, but can be used with roots of other plants.
