Antimicrobial Peptides-Loaded Hydroxyapatite Microsphere With Different Hierarchical Structures for Enhanced Drug Loading, Sustained Release and Antibacterial Activity

Dandan Hong; Dandan Hong; Dandan Hong; Jingjing Wu; Jingjing Wu; Jingjing Wu; Xuemin Xiao; Xuemin Xiao; Xuemin Xiao; Xueyang Li; Xueyang Li; Xueyang Li; Dong Xu; Chang Du; Chang Du; Chang Du; Chang Du

doi:10.3389/fchem.2021.747665

Frontiers in Chemistry (Oct 2021)

Antimicrobial Peptides-Loaded Hydroxyapatite Microsphere With Different Hierarchical Structures for Enhanced Drug Loading, Sustained Release and Antibacterial Activity

Dandan Hong,
Dandan Hong,
Dandan Hong,
Jingjing Wu,
Jingjing Wu,
Jingjing Wu,
Xuemin Xiao,
Xuemin Xiao,
Xuemin Xiao,
Xueyang Li,
Xueyang Li,
Xueyang Li,
Dong Xu,
Chang Du,
Chang Du,
Chang Du,
Chang Du

Affiliations

Dandan Hong: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China
Dandan Hong: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Dandan Hong: Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Jingjing Wu: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China
Jingjing Wu: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Jingjing Wu: Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Xuemin Xiao: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China
Xuemin Xiao: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Xuemin Xiao: Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Xueyang Li: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China
Xueyang Li: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Xueyang Li: Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Dong Xu: Department of Colorectal Surgery, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Chang Du: Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China
Chang Du: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Chang Du: Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China
Chang Du: Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China

DOI: https://doi.org/10.3389/fchem.2021.747665
Journal volume & issue: Vol. 9

Abstract

Read online

Antimicrobial peptides (AMPs) have great potential for clinical treatment of bacterial infection due to the broad-spectrum and highly effective antibacterial activity. However, the easy degradation and inactivation in vivo has been a major obstacle for their application and an effective delivery system is demanding. The surface physicochemical properties of the carrier, including surface potential, surface polarity, pore structure and morphology, have exerted great effects on the adsorption and release behavior of AMPs. This study investigated the influence of micro/nano carriers with different hierarchical structures on the loading, release and biological behavior of AMPs. Three types of AMPs-loaded hydroxyapatite microspheres (HA/AMPs MSs) with different hierarchical structures (needle-like, rod-like, and flake-like) were developed, which was investigated by the surface morphology, chemical composition and surface potential in detail. The different hierarchical structures of hydroxyapatite microspheres (HA MSs) had noticeable impact on the loading and release behavior of AMPs, and the flake-like HA MSs with hierarchical structure showed the highest loading efficiency and long-lasting release over 9 days. Meanwhile, the stability of AMPs released from HA MSs was effectively maintained. Moreover, the antibacterial test indicated that the flake-like HA/AMPs MSs showed more sustained antibacterial properties among three composites. In view of the excellent biocompatibility and osteogenic property, high loading efficiency and the long-term release properties of HA MSs with hierarchical structure, the HA/AMPs MSs have a great potential in bone tissue engineering.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

About the journal

Abstract

Keywords