Characterization of trisubstituted cholanoic acids in human feces

Abstract

The trisubstituted cholanoic acids in human feces have been studied by gas chromatography-mass spectrometry. The following bile acids have been identified: 3Β,7α,12α-trihydroxy-, 3Β,7Β,12α-trihydroxy-, 3α,7α-dihydroxy-12-keto-5Β-cholanoic acids and 3α,7α,12α-trihydroxy-5α-cholanoic acid. The presence in human feces of 3α,7α,12α-trihydroxy-, 3α,7Β,12α-trihydroxy-, and 3α,12α-dihydroxy-7-keto-5Β-cholanoic acids has been confirmed.The composition of bile acids in human feces is summarized and possible metabolic interrelationships suggested.

Keywords

• bile acids
• trifluoroacetates
• partial trimethylsilyl ethers
• dimethylhydrazones
• gas-liquid chromatography
• mass spectrometry