New Antiproliferative Cembrane Diterpenes from the Red Sea <i>Sarcophyton</i> Species
Hossam M. Hassan,
Mostafa E. Rateb,
Marwa H. Hassan,
Ahmed M. Sayed,
Samah Shabana,
Mai Raslan,
Elham Amin,
Fathy A. Behery,
Osama M. Ahmed,
Abdullatif Bin Muhsinah,
Tobias A. M. Gulder,
Usama Ramadan Abdelmohsen
Affiliations
Hossam M. Hassan
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
Mostafa E. Rateb
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
Marwa H. Hassan
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
Ahmed M. Sayed
Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62514, Egypt
Samah Shabana
Pharmacognosy Department, College of Pharmaceutical Sciences & Drug Manufacturing, Misr University for Science and Technology (MUST), Six October City 77, Egypt
Mai Raslan
Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt
Elham Amin
Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
Fathy A. Behery
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
The combination of liquid chromatography coupled to high resolution mass spectrometry (LC-HRESMS)-based dereplication and antiproliferative activity-guided fractionation was applied on the Red Sea-derived soft coral Sarcophyton sp. This approach facilitated the isolation of five new cembrane-type diterpenoids (1−5), along with two known analogs (6 and 7), as well as the identification of 19 further, known compounds. The chemical structures of the new compounds were elucidated while using comprehensive spectroscopic analyses, including one-dimensional (1D) and two-dimensional (2D) NMR and HRMS. All of the isolated cembranoids (1−7) showed moderate in vitro antiproliferative activity against a human breast cancer cell line (MCF-7), with IC50 ranging from 22.39−27.12 µg/mL. This class of compounds could thus serve as scaffold for the future design of anticancer leads.
