Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum
Jie Hu,
Enlai Song,
Yuhui Liu,
Qiaochu Yang,
Junhui Sun,
Jinna Chen,
Yue Meng,
Yanwei Jia,
Zhiguang Yu,
Yang Ran,
Liyang Shao,
Perry Ping Shum
Affiliations
Jie Hu
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Enlai Song
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Yuhui Liu
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Qiaochu Yang
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Junhui Sun
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Jinna Chen
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Yue Meng
Department of Clinical Laboratory, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 511436, China
Yanwei Jia
State-Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, Faculty of Science and Technology-ECE, Faculty of Health Sciences, MoE Frontiers Science Center for Precision Oncology, University of Macau, Macau 999078, China
Zhiguang Yu
Medcaptain Medical Technology Co., Ltd., Shenzhen 518055, China
Yang Ran
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Liyang Shao
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Perry Ping Shum
Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Detection of trace tumor markers in blood/serum is essential for the early screening and prognosis of cancer diseases, which requires high sensitivity and specificity of the assays and biosensors. A variety of label-free optical fiber-based biosensors has been developed and yielded great opportunities for Point-of-Care Testing (POCT) of cancer biomarkers. The fiber biosensor, however, suffers from a compromise between the responsivity and stability of the sensing signal, which would deteriorate the sensing performance. In addition, the sophistication of sensor preparation hinders the reproduction and scale-up fabrication. To address these issues, in this study, a straightforward lasso-shaped fiber laser biosensor was proposed for the specific determination of carcinoembryonic antigen (CEA)-related cell adhesion molecules 5 (CEACAM5) protein in serum. Due to the ultra-narrow linewidth of the laser, a very small variation of lasing signal caused by biomolecular bonding can be clearly distinguished via high-resolution spectral analysis. The limit of detection (LOD) of the proposed biosensor could reach 9.6 ng/mL according to the buffer test. The sensing capability was further validated by a human serum-based cancer diagnosis trial, enabling great potential for clinical use. The high reproduction of fabrication allowed the mass production of the sensor and extended its utility to a broader biosensing field.
