Thermal degradation, antioxidant and antimicrobial activity of the synthesized allicin and allicin incorporated in gel

Abstract

The main carriers of the pharmacological activity of garlic (Allium sativum L.) are organic sulfur compounds, the most important among them being allicin, a sulfenic acid thioester, or allylthiosulfonate. In this paper, the identification of synthesized and purified allicin was determined by using various spectroscopic methods (UV/VIS, FTIR, NMR). A HPLC method was developed for the detection and determination of the allicin content. The thermal degradation of allicin by using FTIR method was monitored. The method for the production of allicin gel based on Carbopol 940 (poly(acrylic acid)) was elaborated. The antimicrobial activity of pure allicin and allicin incorporated into gel by using a disk diffusion method was determined. In order to determine the antioxidant activity of allicin DPPH test was done and it was proved that with low concentrations (1 mgcm-3) a high DPPH radicals scavenging capacity (90%) was achieved.

Keywords

• allicin
• thermal degradation
• antioxidant activity
• antimicrobial activity
• HPLC
• UV/VIS
• FTIR
• NMR