Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site

Jaeyong Lee; Liam J. Worrall; Marija Vuckovic; Federico I. Rosell; Francesco Gentile; Anh-Tien Ton; Nathanael A. Caveney; Fuqiang Ban; Artem Cherkasov; Mark Paetzel; Natalie C. J. Strynadka

doi:10.1038/s41467-020-19662-4

Nature Communications (Nov 2020)

Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site

Jaeyong Lee,
Liam J. Worrall,
Marija Vuckovic,
Federico I. Rosell,
Francesco Gentile,
Anh-Tien Ton,
Nathanael A. Caveney,
Fuqiang Ban,
Artem Cherkasov,
Mark Paetzel,
Natalie C. J. Strynadka

Affiliations

Jaeyong Lee: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Liam J. Worrall: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Marija Vuckovic: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Federico I. Rosell: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Francesco Gentile: Vancouver Prostate Centre, The University of British Columbia
Anh-Tien Ton: Vancouver Prostate Centre, The University of British Columbia
Nathanael A. Caveney: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Fuqiang Ban: Vancouver Prostate Centre, The University of British Columbia
Artem Cherkasov: Vancouver Prostate Centre, The University of British Columbia
Mark Paetzel: Department of Molecular Biology and Biochemistry, Simon Fraser University
Natalie C. J. Strynadka: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia

DOI: https://doi.org/10.1038/s41467-020-19662-4
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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The SARS-CoV-2 main protease (Mpro) is one of two cysteine proteases essential for viral replication. Here, the authors determine the crystal structure of an Mpro acyl intermediate with its native C-terminal autocleavage sequence and the structure of a product bound active site mutant (C145A), which are of interest for antiviral drug development.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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