Frontiers in Cellular and Infection Microbiology (Nov 2022)

Intranasal delivery of a chimpanzee adenovirus vector expressing a pre-fusion spike (BV-AdCoV-1) protects golden Syrian hamsters against SARS-CoV-2 infection

  • Shen Wang,
  • Long Xu,
  • Ting Mu,
  • Mian Qin,
  • Ping Zhao,
  • Liang Xie,
  • Linsen Du,
  • Yue Wu,
  • Nicolas Legrand,
  • Karine Mouchain,
  • Guillaume Fichet,
  • Yi Liu,
  • Yi Liu,
  • Wenhao Yin,
  • Jin Zhao,
  • Min Ji,
  • Bo Gong,
  • Michel Klein,
  • Michel Klein,
  • Ke Wu,
  • Ke Wu

DOI
https://doi.org/10.3389/fcimb.2022.979641
Journal volume & issue
Vol. 12

Abstract

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We evaluated the immunogenicity and protective ability of a chimpanzee replication-deficient adenovirus vectored COVID-19 vaccine (BV-AdCoV-1) expressing a stabilized pre-fusion SARS-CoV-2 spike glycoprotein in golden Syrian hamsters. Intranasal administration of BV-AdCoV-1 elicited strong humoral and cellular immunity in the animals. Furthermore, vaccination prevented weight loss, reduced SARS-CoV-2 infectious virus titers in the lungs as well as lung pathology and provided protection against SARS-CoV-2 live challenge. In addition, there was no vaccine-induced enhanced disease nor immunopathological exacerbation in BV-AdCoV-1-vaccinated animals. Furthermore, the vaccine induced cross-neutralizing antibody responses against the ancestral strain and the B.1.617.2, Omicron(BA.1), Omicron(BA.2.75) and Omicron(BA.4/5) variants of concern. These results demonstrate that BV-AdCoV-1 is potentially a promising candidate vaccine to prevent SARS-CoV-2 infection, and to curtail pandemic spread in humans.

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