SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

Steffen Klein; Mirko Cortese; Sophie L. Winter; Moritz Wachsmuth-Melm; Christopher J. Neufeldt; Berati Cerikan; Megan L. Stanifer; Steeve Boulant; Ralf Bartenschlager; Petr Chlanda

doi:10.1038/s41467-020-19619-7

Nature Communications (Nov 2020)

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

Steffen Klein,
Mirko Cortese,
Sophie L. Winter,
Moritz Wachsmuth-Melm,
Christopher J. Neufeldt,
Berati Cerikan,
Megan L. Stanifer,
Steeve Boulant,
Ralf Bartenschlager,
Petr Chlanda

Affiliations

Steffen Klein: Schaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg University
Mirko Cortese: Department of Infectious Diseases-Molecular Virology, Heidelberg University
Sophie L. Winter: Schaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg University
Moritz Wachsmuth-Melm: Schaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg University
Christopher J. Neufeldt: Department of Infectious Diseases-Molecular Virology, Heidelberg University
Berati Cerikan: Department of Infectious Diseases-Molecular Virology, Heidelberg University
Megan L. Stanifer: Department of Infectious Diseases-Molecular Virology, Heidelberg University
Steeve Boulant: Research Group “Host/Pathogen Interactions at Intestinal Epithelial Surfaces,” Department of Infectious Diseases, Virology, Heidelberg University
Ralf Bartenschlager: Department of Infectious Diseases-Molecular Virology, Heidelberg University
Petr Chlanda: Schaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg University

DOI: https://doi.org/10.1038/s41467-020-19619-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

Here the authors visualize SARS-CoV-2 infected cells by in situ cryo-electron tomography, delineating the structural organization and conformational changes that occur during virus replication and budding; and provide insight into vRNP architecture and RNA networks in double membrane vesicles.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal