Nanotechnology, Science and Applications (Apr 2022)
Parallel Multichannel Assessment of Rotationally Manipulated Magnetic Nanoparticles
Abstract
Syed I Hussain,1– 3 Lamar O Mair,4 Alexander J Willis,5 Georgia Papavasiliou,2 Bing Liu,6 Irving N Weinberg,4 Herbert H Engelhard1,3,7 1Department of Neurosurgery, The University of Illinois at Chicago, Chicago, IL, USA; 2Biomedical Engineering Department, Illinois Institute of Technology, Chicago, IL, USA; 3NanoMagnetic Therapeutics Corp., Wilmette, IL, USA; 4Weinberg Medical Physics, Inc., North Bethesda, MD, USA; 5Department of Medicine, The University of Illinois at Chicago, Chicago, IL, USA; 6IMRA America, Inc., Ann Arbor, MI, USA; 7Department of Bioengineering, The University of Illinois at Chicago, Chicago, IL, USACorrespondence: Herbert H Engelhard, Email [email protected]: Rotational manipulation of chains or clusters of magnetic nanoparticles (MNPs) offers a means for directed translation and payload delivery that should be explored for clinical use. Multiple MNP types are available, yet few studies have performed side-by-side comparisons to evaluate characteristics such as velocity, movement at a distance, and capacity for drug conveyance or dispersion.Purpose: Our goal was to design, build, and study an electric device allowing simultaneous, multichannel testing (e.g., racing) of MNPs in response to a rotating magnetic field. We would then select the “best” MNP and use it with optimized device settings, to transport an unbound therapeutic agent.Methods: A magnetomotive system was constructed, with a Helmholtz pair of coils on either side of a single perpendicular coil, on top of which was placed an acrylic tray having multiple parallel lanes. Five different MNPs were tested: graphene-coated cobalt MNPs (TurboBeads™), nickel nanorods, gold-iron alloy MNPs, gold-coated Fe3O4 MNPs, and uncoated Fe3O4 MNPs. Velocities were determined in response to varying magnetic field frequencies (5– 200 Hz) and heights (0– 18 cm). Velocities were normalized to account for minor lane differences. Doxorubicin was chosen as the therapeutic agent, assayed using a CLARIOstar Plus microplate reader.Results: The MMS generated a maximal MNP velocity of 0.9 cm/s. All MNPs encountered a “critical” frequency at 20– 30 Hz. Nickel nanorods had the optimal response based on tray height and were then shown to enable unbound doxorubicin dispersion along 10.5 cm in < 30 sec.Conclusion: A rotating magnetic field can be conveniently generated using a three-coil electromagnetic device, and used to induce rotational and translational movement of MNP aggregates over mesoscale distances. The responses of various MNPs can be compared side-by-side using multichannel acrylic trays to assess suitability for drug delivery, highlighting their potential for further in vivo applications.Keywords: electromagnetic field, doxorubicin, drug delivery, magnetic drug targeting, nanoparticle assay, rotating fields
