Herbicidal secondary metabolites from Bacillus velezensis JTB8-2 against Orobanche aegyptiaca
Wei He,
Yan Li,
Wenfang Luo,
Junhui Zhou,
Sifeng Zhao,
Jianjun Xu
Affiliations
Wei He
Xinjiang Production and Construction Corps, Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Key Laboratory, Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Agriculture College of Shihezi University
Yan Li
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences
Wenfang Luo
Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences
Junhui Zhou
Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences
Sifeng Zhao
Xinjiang Production and Construction Corps, Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Key Laboratory, Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Agriculture College of Shihezi University
Jianjun Xu
Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences
Abstract Egyptian broomrape (Orobanche aegyptiaca) is a parasitic plants that cause significant losses to important crops. The effective methods for controlling this weed are rare. Biological control could be one of the possible strategies to tackle these weeds efficiently. In this work, a bacteria strain Bacillus velezensis JTB8–2 was proven to possesse biological control functions against broomrapes in both pot and field experiments. Four secondary metabolites (1–4) were isolated from the B. velezensis JTB8–2 crude extracts, and all of them could inhibit the germination of O. aegyptiaca seeds at concentrations from 0.5 mM to 4 mM. Their structures were further elucidated by Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) analysis. Among the isolated compounds, 1 and 2 exhibited the strongest herbicidal activity with 100% inhibition rate against the germination of O. aegyptiaca seeds at 4 mM, and thus had great potential in the development of new herbicidal products to control O. aegyptiaca in the future.
