玉米肽对大鼠酒精性脑损伤的保护作用

Abstract

为研发具有预防酒精性脑损伤功效的天然活性肽类产品，将SD大鼠随机分为7组，分别为对照组、模型组、玉米肽实验组(剂量分别为125、250、500、1 000、2 000 mg/kg)，采用50度北大仓部优白酒灌胃建立大鼠慢性酒精中毒脑损伤模型，造模结束后取脑组织进行病理组织学观察，同时采用酶联免疫试剂盒检测各组大鼠脑组织中氧化应激、炎性因子、部分神经递质及其他相关指标。结果表明：酒精可使脑组织神经细胞发生病理性改变，玉米肽干预可有效缓解酒精对脑组织的损伤作用；玉米肽能够减少酒后大鼠脑组织中ROS含量，提高GSH的含量，恢复GST和GSH-Px活性；玉米肽可降低脑组织中NF-κB含量，减少IL-1、TGF-β1、TNF-α和IFN-γ的分泌；玉米肽对酒精诱导的脑组织中Caspase-3的激活有积极影响，对慢性酒精中毒大鼠脑组织中部分神经递质有一定的调节作用。综上，玉米肽对大鼠慢性酒精性脑损伤具有保护作用。 In order to develop the natural active peptide with the function of preventing alcoholic brain damage, SD rats were randomly divided into 7 groups, control group, model group, and 125, 250, 500, 1 000 mg/kg and 2 000 mg/kg corn peptide experimental groups, and the chronic alcoholism brain damage model in rats was established by administration of 50% Beidachang liquor. After modelling, the brain tissue was taken for histopathological observation and ELISA mensuration, including oxidative stress, inflammatory cytokines levels, some neurotransmitters and other related indicators. The results showed that alcohol could induce pathological changes of nerve cells in brain tissue, and the intervention of corn peptide could effectively relieve the damage of brain tissue. Corn peptide could reduce ROS and improve GSH levels, recover GST and GSH-Px activity in brain tissue of rats after drinking, and reduce the levels of NF-κB, TNF-α, IL-1, IFN-γ and TGF-β1 in brain tissue. Corn peptide also had positive effects on the activation of Caspase-3 and regulate some neurotransmitters in brain tissue of rats with chronic alcoholism. In conclusion, corn peptide has protective effects on chronic alcoholism brain damage in rats.

Keywords

• 玉米肽；酒精；脑损伤
• corn peptide; alcohol; brain damage