Dynamics of laser speckle imaging of blood flow in the tissues at partial temporary pancreatic ischemia of hungry, fed and alcoholized rats

Abstract

The aim of the study is to determine the influence of a partial temporary ischemia and reperfusion on the development of pancreatitis in laboratory rats. Material and Methods. The experiments were performed on 73 white rats. Occluded blood vessels and blood flow were recorded by digital biomicroscopy and laser speckle contrast imaging. Statistical data processing was carried out the program Statistica for Windows 6.0 using the Student's criterion. Results. Laser speckle imaging and digital microscopes provide the monitoring of blood flow in the vessels of the pancreas. After a five-minute partial ischemia reactive hyperemia occurs, changes are invertible. After a twenty-minute partial ischemic reactive hyperemia does not occur, without developing pancreatitis necrosis. Pancreas of alcoholized rats has been found out to be more sensitive to ischemia. Conclusion. The method of laser speckle imaging and intravital microscopy after digital five- and twenty-minute partial ischemia of the pancreas revealed phase opposite directed changes in its tissue perfusion and varying severity of postischemic pancreatitis.

Keywords

• intravital microscopy
• pancreas
• reperfusion
• speckle imaging