The Effect of Different Water Extracts from <i>Platycodon grandiflorum</i> on Selected Factors Associated with Pathogenesis of Chronic Bronchitis in Rats
Waldemar Buchwald,
Michał Szulc,
Justyna Baraniak,
Natalia Derebecka,
Małgorzata Kania-Dobrowolska,
Anna Piasecka,
Anna Bogacz,
Monika Karasiewicz,
Joanna Bartkowiak-Wieczorek,
Radosław Kujawski,
Agnieszka Gryszczyńska,
Piotr Kachlicki,
Mariola Dreger,
Marcin Ożarowski,
Anna Krajewska-Patan,
Małgorzata Górska-Paukszta,
Ewa Kamińska,
Przemysław Ł. Mikołajczak
Affiliations
Waldemar Buchwald
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Michał Szulc
Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland
Justyna Baraniak
Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Natalia Derebecka
Laboratory of High Throughput Technologies, Institute of Molecular Biology and Biotechnology, Faclty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
Małgorzata Kania-Dobrowolska
Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Anna Piasecka
Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland
Anna Bogacz
Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Monika Karasiewicz
Laboratory of International Health, Department of Preventive Medicine, Poznań University of Medical Sciences, Święcickiego 6, 60-781 Poznań, Poland
Joanna Bartkowiak-Wieczorek
Department of Physiology, Poznań University of Medical Sciences, Święcickiego 6, 60-781 Poznań, Poland
Radosław Kujawski
Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland
Agnieszka Gryszczyńska
Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Piotr Kachlicki
Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland
Mariola Dreger
Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland
Marcin Ożarowski
Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland
Anna Krajewska-Patan
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Małgorzata Górska-Paukszta
Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland
Ewa Kamińska
Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland
Przemysław Ł. Mikołajczak
Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland
The aim of this study was to assess the activity of extracts from Platycodon grandiflorum A. DC (PG) in a model of chronic bronchitis in rats. The research was carried out on three water extracts: E1 – from roots of field cultivated PG; E2 – from biotransformed roots of PG; E3 – from callus of PG. The extracts differed in saponins and inulin levels—the highest was measured in E3 and the lowest in E1. Identification of secondary metabolites was performed using two complementary LC-MS systems. Chronic bronchitis was induced by sodium metabisulfite (a source of SO2). Animals were treated with extracts for three weeks (100 mg/kg, intragastrically) and endothelial growth factor (VEGF), transforming growth factors (TGF-β1, -β2, -β3), and mucin 5AC (MUC5AC) levels were determined in bronchoalveolar lavage fluid, whereas C reactive protein (CRP) level was measured in serum. Moreover, mRNA expression were assessed in bronchi and lungs. In SO2-exposed rats, an elevation of the CRP, TGF-β1, TGF-β2, VEGF, and mucin was found, but the extracts’ administration mostly reversed this phenomenon, leading to control values. The results showed a strong anti-inflammatory effect of the extracts from PG.
