Drosophila suzukii and Drosophila melanogaster prefer distinct microbial and plant aroma compounds in a complex fermented matrix
Maria C. Dzialo,
Somasundar Arumugam,
Supinya Piampongsant,
Lloyd Cool,
Christophe Vanderaa,
Beatriz Herrera-Malaver,
Tomas Opsomer,
Wim Dehaen,
Tom Wenseleers,
Miguel Roncoroni,
Amani Alawamleh,
Felix Wäckers,
Bart Lievens,
Bill S. Hansson,
Karin Voordeckers,
Silke Sachse,
Kevin J. Verstrepen
Affiliations
Maria C. Dzialo
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium
Somasundar Arumugam
Research Group Olfactory Coding, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str, 8, 07745 Jena, Germany; Max Planck Center Next Generation Insect Chemical Ecology (nGICE), Hans-Knoell-Str, 8, 07745 Jena, Germany
Supinya Piampongsant
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium
Lloyd Cool
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium; Laboratory of Socioecology and Social Evolution, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium
Christophe Vanderaa
Laboratory of Socioecology and Social Evolution, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium
Beatriz Herrera-Malaver
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium
Tomas Opsomer
Sustainable Chemistry for Metals and Molecules, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium
Wim Dehaen
Sustainable Chemistry for Metals and Molecules, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium
Tom Wenseleers
Laboratory of Socioecology and Social Evolution, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium
Miguel Roncoroni
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium
Amani Alawamleh
Biobest NV, Ilse Velden 18, 2260 Westerlo, Belgium; University of Molise, Department of Agricultural, Environmental and Food Sciences, Via De Sanctis 1, 86100 Campobasso, Italy
Felix Wäckers
Biobest NV, Ilse Velden 18, 2260 Westerlo, Belgium; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK
Bart Lievens
CMPG Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Department M2S, KU Leuven, Willem De Croylaan 46, 3001 Leuven, Belgium
Bill S. Hansson
Max Planck Center Next Generation Insect Chemical Ecology (nGICE), Hans-Knoell-Str, 8, 07745 Jena, Germany; Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str, 8, 07745 Jena, Germany
Karin Voordeckers
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium
Silke Sachse
Research Group Olfactory Coding, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str, 8, 07745 Jena, Germany; Max Planck Center Next Generation Insect Chemical Ecology (nGICE), Hans-Knoell-Str, 8, 07745 Jena, Germany; Corresponding author
Kevin J. Verstrepen
VIB – KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001 Leuven, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Gaston Geenslaan 1, 3001 Leuven, Belgium; Corresponding author
Summary: Volatile aroma compounds are important chemical cues for insects. Behavioral responses to specific odors differ strongly between insect species, and the exact causative molecules are often unknown. Beer is frequently used in insect traps because it combines hundreds of plant and microbial aromas that attract many insects. Here, we analyzed responses of the pest fruit fly Drosophila suzukii and benign Drosophila melanogaster to beers with different chemical compositions. Using extensive chemical and behavioral assays, we identified ecologically relevant chemicals that influence drosophilid behavior and that induce different odor-evoked activity patterns in the antennal lobe of the two species obtained by functional imaging. Specific mixes of compounds increased the species-specificity and sex-specificity of lures in both laboratory and greenhouse settings. Together, our study shows how examining insect responses to highly complex natural mixtures of aroma compounds provides insight into insect-specific behavioral responses and also opens avenues for improved pest control.
