Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)

Abstract

Summary: Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach.For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.

Keywords

• Cell Biology
• Molecular/Chemical Probes
• Protein Biochemistry
• Biotechnology and bioengineering