Comparing the growth and the development of mouse pre-antral follicle in medium with PL (Platelet Layset) and with FBS

Abstract

Introduction: In recent years a great deal of effort has been made to improve the culture medium which can be used for different cell culturing and Folliculogenesis. PL (Platelet Layset) with a high percentage of growth factors and also microelements can be effective on the growth of follicle and oocyte. Material and method: Preantral follicles were collected from 12 to 14 days female NMRI mice and cultured in culture medium with a different supplement as the serum for 12 days. The different serums were, 5% and 10% FBS as the control groups and the experimental groups were enriched by 5% PL, 10% PL and a combination of 5% PL and 5% FBS. The growth and development of follicle and oocyte was monitored and also the amount of Estradiol (E2) and progesterone (P4) was detected in different mediums on days 9 and 12 of culturing. Result: Oocytes had a significant growth in all medium compared to day zero and reached to the size of a mature oocyte (p < 0.05). Although survival rate after one day had significant decrease in all medium compared to 5% FBS (p < 0.05), after 12 days of culturing, the best survival rate belonged to 5% PL. Progesterone secretion increased in experimental groups significantly (p < 0.05) while Estradiol secretion in 5% and 10% PL decreased in half of control groups. Discussion: This result demonstrates that PL can be a considerable supplement that can be added to the culture medium or a good replacement for FBS as serum used in Folliculogenesis.

Keywords

• In Vitro Folliculogenesis
• Platelet Layset (PL)
• In Vitro Fertilization (IVF)
• Pre-antral follicle
• Fetal Bovine Serum (FBS)