Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

Sandip A. Shelke; Yaming Shao; Artur Laski; Deepak Koirala; Benjamin P. Weissman; James R. Fuller; Xiaohong Tan; Tudor P. Constantin; Alan S. Waggoner; Marcel P. Bruchez; Bruce A. Armitage; Joseph A. Piccirilli

doi:10.1038/s41467-018-06942-3

Nature Communications (Oct 2018)

Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

Sandip A. Shelke,
Yaming Shao,
Artur Laski,
Deepak Koirala,
Benjamin P. Weissman,
James R. Fuller,
Xiaohong Tan,
Tudor P. Constantin,
Alan S. Waggoner,
Marcel P. Bruchez,
Bruce A. Armitage,
Joseph A. Piccirilli

Affiliations

Sandip A. Shelke: Department of Biochemistry and Molecular Biology, The University of Chicago
Yaming Shao: Department of Biochemistry and Molecular Biology, The University of Chicago
Artur Laski: Department of Biochemistry and Molecular Biology, The University of Chicago
Deepak Koirala: Department of Biochemistry and Molecular Biology, The University of Chicago
Benjamin P. Weissman: Department of Chemistry, The University of Chicago
James R. Fuller: Department of Biochemistry and Molecular Biology, The University of Chicago
Xiaohong Tan: Center for Nucleic Acids Science and Technology, Carnegie Mellon University
Tudor P. Constantin: Department of Chemistry, Carnegie Mellon University
Alan S. Waggoner: Department of Biological Sciences, Carnegie Mellon University
Marcel P. Bruchez: Department of Chemistry, Carnegie Mellon University
Bruce A. Armitage: Center for Nucleic Acids Science and Technology, Carnegie Mellon University
Joseph A. Piccirilli: Department of Biochemistry and Molecular Biology, The University of Chicago

DOI: https://doi.org/10.1038/s41467-018-06942-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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