Establishment and application of a PCR assay for the identification of virulent and attenuated duck plague virus DNA in cotton swabs

Ying Wu; Shanshan Zhang; Yuqi Li; Chenyang Pan; Mingshu Wang; Shun Chen; Renyong Jia; Qiao Yang; Dekang Zhu; Mafeng Liu; Xinxin Zhao; Shaqiu Zhang; Juan Huang; Xumin Ou; Sai Mao; Qun Gao; Di Sun; Bin Tian; Anchun Cheng

Poultry Science (May 2023)

Establishment and application of a PCR assay for the identification of virulent and attenuated duck plague virus DNA in cotton swabs

Ying Wu,
Shanshan Zhang,
Yuqi Li,
Chenyang Pan,
Mingshu Wang,
Shun Chen,
Renyong Jia,
Qiao Yang,
Dekang Zhu,
Mafeng Liu,
Xinxin Zhao,
Shaqiu Zhang,
Juan Huang,
Xumin Ou,
Sai Mao,
Qun Gao,
Di Sun,
Bin Tian,
Anchun Cheng

Affiliations

Ying Wu: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Shanshan Zhang: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Yuqi Li: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Chenyang Pan: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Mingshu Wang: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Shun Chen: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Renyong Jia: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Qiao Yang: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Dekang Zhu: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Mafeng Liu: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Xinxin Zhao: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Shaqiu Zhang: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Juan Huang: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Xumin Ou: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Sai Mao: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Qun Gao: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Di Sun: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Bin Tian: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China
Anchun Cheng: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, China; Corresponding author:

Journal volume & issue: Vol. 102, no. 5
p. 102555

Abstract

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ABSTRACT: Duck plague is an acute, febrile, and septic infectious disease caused by duck plague virus (DPV), which causes serious harm to the duck industry in China. Ducks latently infected with DPV display a clinically healthy state, which is one of the epidemiological characteristics of duck plague. In the present study, to rapidly distinguish vaccine-immunized ducks from wild virus-infected ducks during production, a PCR assay based on the newly identified LORF5 fragment was developed to effectively and accurately identify viral DNA in cotton swab samples and was used to assess artificial infection models and clinical samples. The results showed that the established PCR method had good specificity and that only the virulent and attenuated DNA of duck plague virus was specifically amplified, as the results for the detection of common duck pathogens (duck hepatitis B virus, duck Tembusu virus, duck hepatitis A virus type 1, novel duck reovirus, Riemerella anatipestifer, Pasteurella multocida, and Salmonella) were negative. The amplified fragments of virulent and attenuated strains were 2,454 bp and 525 bp, and their minimum detection amounts were 0.46 pg and 46 pg, respectively. The detection rate of the virulent and attenuated DPV strains in duck oral and cloacal swabs was lower than that of the gold standard PCR method (GB-PCR, which is unable to distinguish virulent and attenuated strains), and cloacal swabs from clinically healthy ducks were more suitable for detection than oral swabs. In conclusion, the PCR assay established in the present study can be used as a simple and effective method for the clinical screening of ducks that are latently infected with virulent strains of DPV and shedding virus, which can provide technical support for the elimination of duck plague from duck farms.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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